MFHPB-02 Method for the Direct Microscopic Examination of Foods in Hermetically Sealed Containers
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1E9C4A8D76414A1D99A40A75AABC6FA9 |
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文件大小(MB)： |
0.03 |
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页数： |
6 |
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日期： |
2012-3-2 |
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Published on the Food Directorate’s (Health Canada's) website at,http://www.hc-sc.gc.ca/fn-an/res-rech/analy-meth/microbio/index_e.html,Government of Canada Gouvernement du Canada,HPB Method MFHPB-02,October 2006,HEALTH PRODUCTS AND FOOD BRANCH,OTTAWA,METHOD FOR THE DIRECT MICROSCOPIC EXAMINATION OF FOODS,IN HERMETICALLY SEALED CONTAINERS,Alexander Fabricius,Fish/Food Laboratory,Canadian Food Inspection Agency,Mississauga, Ontario, L5T 2R4,E-mail: fabriciusa@inspection.gc.ca,Patti Wilson,CFIA Dartmouth Laboratory,1992 Agency Dr,Dartmouth, NS, B3B 1Y9,E-mail: wilsonpa@inspection.gc.ca,Microbiological Methods Committee,Health Products and Food Branch,Microbiology Evaluation Division,Bureau of Microbial Hazards, Food Directorate,Postal Locator 2204A1,Ottawa, Ontario, K1A 0L2,E-mail: Don_Warburton@hc-sc.gc.ca,Dev C. Nundy,Food Laboratory,Laboratory Services Division,Canadian Food Inspection Agency,Ottawa, Ontario, K1A 0C6,E-mail: dnundy@inspection.gc.ca,1. APPLICATION,This method can be used for the microscopic examination of incipient or non-incipient type spoiled foods in,thermally processed hermetically sealed containers, that are suspected of being compromised,microbiologically . Ascertaining morphological characteristics of organisms is necessary for the purposes of,determining whether it has public health significance or not. This revised method replaces MFHPB-25G,dated October 1991.,2. PRINCIPLE,The method describes the microbiologically suspect contaminated canned foods or other hermetically sealed,containers that may be used in the preparation of simple stained smears and wet mounts, their microscopic,examination and recording of results.,NOTE: If the analyst uses any variations of the staining procedures listed here (either product that is,commercially available or made from scratch), it is the responsibility of the analyst or Laboratory,Supervisor to ensure equivalency.,3. DEFINITION OF TERMS,- 2 - MFHPB-02,October 2006,See Appendix A of Volume 2 for definitions not covered in this section.,3.1 Hermetically Sealed Containers: These are containers designed to be secure against the entry of air,and microorganisms into the food product. These containers may be: 1) rigid, such as glass bottles,and two or three piece metal cans; 2) semi-rigid, such as disposable serving dishes used to contain,some ready-to-eat meals, and 3) flexible, such as retort pouches.,3.2 Semi-Liquid Products: These are products that may pour with difficulty, but can be mixed by shaking,the container.,3.3 Semi-Solid Products: These are products that have a high viscosity; they pour with great difficulty,and cannot be mixed by shaking the unopened container.,3.4 Acid and Acidified Food: a food with a pH equal to or lower than 4.6.,4. COLLECTION OF SAMPLES,4.1 See Appendix B of Volume 2 and follow instructions, if applicable. Depending on the circumstance,(container type, food type and the number of samples requiring microscopic examination), containers,may be examined individually.,5. MATERIALS AND SPECIAL EQUIPMENT,1) All materials and special equipment specific to the aseptic analysis of foods in hermetically sealed,containers, as listed in MFHPB-01,2) Sterile bacteriological sampling loops, applicator stick, transfer pipette or other appropriate utensil for,obtaining a smear sample,3) Light microscope with a 100 x oil immersion objective,4) Microbiological dyes, such as ethanol based solutions of crystal violet or methylene blue,5) Xylol,6) Clean microscope slides and cover slips,7) 0.05N Sodium hydroxide (NaOH) solution,6. SAFETY PRECAUTIONS,HANDLE ALL SWOLLEN CONTAINERS AS IF THEY CONTAINED CLOSTRIDIUM BOTULINUM AND/OR,THEIR TOXINS UNTIL THE ABSENCE OF SUCH HAS BEEN ESTABLISHED.,NEVER TASTE FOOD FROM A CAN UNDER INVESTIGATION. ALWAYS USE MECHANICAL PIPETTING,DEVICES.,SWOLLEN CONTAINERS, ESPECIALLY HARD SWELLS, CAN SPRAY THEIR CONTENTS UPON BEING,PUNCTURED. TO PREVENT CONTAMINATION OF SURROUNDING ENVIRONMENT, SUITABLE,HANDLING PRACTICES MUST BE USED. IT IS RECOMMENDED THAT SWOLLEN CONTAINERS BE,- 3 - MFHPB-02,October 2006,HANDLED IN A CLASS II BIOSAFETY CABINET WHICH PROVIDES PROTECTION TO THE OPERATOR,AS WELL AS THE PRODUCT.,NEVER FLAME HARD SWELLS SINCE THE ABSORBED HEAT MAY INDUCE THE CANS TO BURST.,DYES AND SOLVENTS CAN BE HARMFUL. REVIEW MATERIAL SAFETY DATA SHEETS AND USE,PERSONAL PROTECTIVE EQUIPMENT AS APPROPRIATE.,7. PROCEDURE,7.1 Handling and Preparation of sample units,7.1.1 Follow MFHPB-01, for the identification of the container, removal and identification of the,label, aseptic opening of the container, and mixing the contents (as applicable).,7.2 Preparation of a dry smear,Try, wher……
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